【摘要】 用SRBC经不同途径(sc+ip、sc+iv、ip、iv)免疫单只动物即可诱导产生迟发型变态反应(DTH),又能同时诱导产生抗体,皮下(sc)免疫只能诱导产生DTH。这个动物模型的灵敏性用免疫抑制药物坏磷酰胺和免疫增强药物厌氧棒状杆菌菌苗进行检测。给予小鼠环磷酰胺可明显抑制DTH和抗体(IgM PFC)的反应,而厌氧棒状杆菌菌苗可明显增强小鼠的抗体反应。多项免疫功能检测与单项免疫功能检测方法所得结果非常相似,单个动物多项免疫功能检测是一个经济、省时、灵敏的评价化合物和药物的免疫毒性和药理毒性的方法。更多还原

【Abstract】 Mice were immunized with SRBC by different ways (sc. +ip., sc. +iv.,ip., iv.) to induce boththe delayed type bypersensitivity (DTH) and specific antibody responses in a single animal. The micewere injected SRBC subcutaneously (sc) only to induce the DTH production. The sensitivity of thisanimal model was tested by treating the mice with the immunesuppressive drug, cyclophosphamide andthe immune-potentiating drug, corynebacterium parvam. The mice treated with cyclophosphamide hadtheir DTH and antibody (IgMPFC) responses decreased while those treated with corynebacteriumparvam had their antibody responses enhanced. The results of multiple immunoassay in the one andthe same mouse were very similar to the results of single immunoassay. The multiple immunoassay ina single animal was an economical, time-saving, sensitive for assessing immunotoxicologic/pharmcolo-gic properties of chemicals and drugs.更多还原