【摘要】 豚鼠淋巴结单个核细胞体外单层培养形成CFU—F集落,其种植的细胞数与成集落数呈良好的线性关系;淋巴结基质祖细胞的Do值为2.8Gy,n值1.5;低浓度(10μg/ml)PHA对集落形成有一定的刺激效应,高浓度(40μg/ml)可抑制集落的形成;经对集落大小、所含细胞数及密度进行比较,说明CFU—F是非均质的,可能因存在不同的亚群。更多还原

【Abstract】 Single nuclear cells derived from lymph nodes of guinea pigs could form CFU-F colonies in monolayer culture system, and there was clearly a linear relationship between the number of implanted cells and the formed colonies.The Do value of the progenitor cells of lymph node was 2.8 Gy,and the n value 1.5. Low concentration (10μg/ml) of PHA might stimulate, while high concentration (40μg/ml) inhibit formation of the colonies. Analysis on the size, number and density of the colonies demonstrated that the CFU-F thus formed were nonhomogenous, and might be heterogeneous subgroups.更多还原