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血小板浓缩液中短链脂肪酸的气相色谱研究

Study of Short Chain Fatty Acids in Platelet Concentrate with Gas Chromatography

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【作者】 包娜然卯新民聂慧芳

【Author】 Bao Naran Mao Xinmin (Institute of Hematology,Lan-zhou Medical College) Nie Huifang(Red Cross Blood Center,Gansu Province)

【机构】 兰州医学院血液学研究所兰州医学院血液学研究所甘肃省红十字血液中心

【摘要】 本文采用气相色谱法测定了血小板浓缩液和血清中的短链脂肪酸。所测短链脂肪酸包括乙酸、丙酸、丁酸、异丁酸、戊酸、异戊酸、乳酸、丙酮酸和琥珀酸。血小板浓缩液中主要含有乙酸、乳酸、丙酮酸和珀琥酸;相对含量分别为1.14、13.50、1.41和9.54。血清中含有乙酸、乳酸和丙酮酸,相对含量分别为0.98、4.33和1.88。血清中未测到琥珀酸,血小板浓缩液中的琥珀酸可以认为主要是血小板的内容物。血小板计数与乳酸含量成正相关关系。取同体积室温保存0、24、48和72小时的血小板浓缩液进行测定,随着保存时间的延长,乳酸含量增加。

【Abstract】 The short chain fatty acids inplatelet concentrate (PC) and serumwere determined by means of gaschromatography.The short chainfatty acids determined includeacetic,propionic,butyric,isobuty-ric,valeric,isovaleric,lactic,pyruvic and succinic acids.Therewere acetic,lactic,pyruvic andsuceinic acids in PC;their relativecontents were 1.14,13.50,1.41 and9.54,respectively.The serum cont-ained acetic,lactic and pyruvic aci-ds;their relative contents were0.98,4.33 and 1.88,respectively.Succinic acid wasn’t found in theserum,suggesting that the succinicacid in PC is the content of platelets.There was a positive correlationbetween the platelet count and lacticacid content of PC.Fresh PC wascompared with the PC of same volumepreserved for 0,24,48,72 hours atroom temperature.The lactic acidcontent of PC increased with thetime of preservation.

  • 【文献出处】 中国输血杂志 ,Chinese Journal of Blood Transfusion , 编辑部邮箱 ,1991年03期
  • 【被引频次】6
  • 【下载频次】242
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