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用电镜技术证明伍氏游仆虫大核染色体DNA端粒含倒转重复序列
PRESENCE OF INVERTED REPEATS IN THE TELOMERE OF MACRONUCLEAR DNA OF EUPLOTES WOODRUFFI
【摘要】 游仆虫(Euplotes)大核的基因组由数以千计的染色体片段组成,其染色体DNA均为20kbp以下的小分子,经变性——局部复性处理和DNA大分子展层,在电镜下显示为单链环形结构,从而直观地证明了染色体DNA端粒序列含有倒转重复序列。
【Abstract】 Chromosomal DNAs of macronuclear genome in Euplotes woodruffi are small molecules below 20 Kbp in size. After denaturation-partial renaturation and spreading of macronuclear DNA, circling of single strand DNA molecules were observed by electronmicroscopy. This confirmes the presence of inverted repeats in the termini (telomeres) of chcromosomal DNA.
- 【文献出处】 动物学报 ,Acta Zoologica Sinica , 编辑部邮箱 ,1991年01期
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