【摘要】 <正> 用小鼠腹腔细胞等作为滋养细胞培养人杂交瘤,国内外均有报道。我们曾建立了人包皮传代细胞株。本文利用我们所建立的人包皮细胞做滋养细胞,并与小鼠腹腔细胞及不加滋养细胞的空白作对照,观察人包皮细胞对杂交瘤克隆生长的影响。 将人包皮细胞和小鼠腹腔细胞分别接种同一96孔板,次日将两株不同的人杂交瘤细胞1B4及1C8稀释至每毫升含10个或50个细胞,每孔接种0.1ml,使每孔分别含1个及5个细胞,经不同时间观察每孔杂交瘤克隆的生长情况,记录有杂交瘤生长的孔数。表1结果表明,更多还原

【Abstract】 The human foreskin(HF ) continous cell line was developed in our laboratory previously. In this paper the HF cells were used as a feeder layer in the cloning of hybridoma, and compared with mouse peritoneal exudate cells as a feeder.Human hybridoiua cells 1B4 and lC8 were diluted into one to five cells for each well on 9G-well plate. The result showed that the number of growth wells of human hybridoma on HF cell feeder layer is much higher than that of mouse peritoneal exudate cell at 5,8, 10 and 15 days after cloning. This indicates that HF cells can be used as a feeder layer for the cloning of human hybridoma, and it has enhancing effect on the growth of cloned cells, much better than mouse peritoneal cxudate cells.更多还原