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稻瘟菌的胞外纤维素酶和果胶酶活性的研究

Activity of cellulase and pectinase in peripery of Piricularia Oryzae Cavara

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【作者】 游树鹏; 韩献忠; 徐礼根; 金逸民;

【Author】 You Shupeng

【机构】 杭州大学; 杭州大学;

【摘要】 液体培养5种稻瘟菌生理小种.随着培养天数增加,ZC3、ZD1和ZG1培养液中纤维素酶活性增高较快,而ZE1和ZF1增高较慢.培养液中纤维素酶活性增高快的,葡萄糖含量增加也快.培养8天的稻瘟菌培养液中各生理小种果胶酶活性高低顺序,果胶甲酯酶为:ZD1>ZC3>ZG1>ZE1>ZF1;多聚半乳糖醛酸酶活性为:ZD1>ZC3>ZE1>ZF1>ZG1.研究表明,胞外纤维素酶和果胶酶活性高的稻瘟菌生理小种,其致病性也强.

【Abstract】 The Cellulase activity and content of glucose of Piricularia OryzaeCavara in races ZC3, ZD1, ZE1, ZF1 and ZG1 in liquid culture were determinedafter different culture days The results were shown as follows: (1) Cellulaseactivity of P. O. Cavara in races ZC3, ZD1and ZG1was higher than that of P. O. Ca-vara in races ZE1, ZF1; (2)The content of glucose in culture solution was higherin accordance with higher cellulase activity; (3)Cellulase activity decreased,but the content of glucose increased significantly after 20 days. Pectin methy-lesterase activity of 5 races in liquid cultrue after 8 days was; ZD1>ZC3>ZG1>ZE1>ZF1. There were positive correlations between the pathogenicity of P.O. Carara and the activity of cellulase and pectinase.

【关键词】 稻瘟菌; 纤维素酶; 果胶酶; 活性;
【Key words】 Piricularia Oryzae Cavara; Pectinase; Cellulase; activity;
  • 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,1991年04期
  • 【被引频次】12
  • 【下载频次】152
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