【摘要】 双酚胺酸对铀有良好的促排解毒作用,但它却有明显的胚胎毒和致畸作用。用接近治疗的剂量100,250,500mg/kg肌注,引起吸收胎明显增多,甚至达100％。在不同剂量组幸存的鼠活胎中有3.3-6.9％的畸胎。双酚胺酸无诱变作用。在CHL细胞染色体畸变试验中浓度高达LD50时,畸变率仍低于5％。ICR小鼠肌注3000mg/kg后,骨髓多染性红细胞微核率无明显增高。进一步分析双酚胺酸对DNA构象的影响。结果50μg/ml双酚胺酸并不改变小牛胸腺DNA的圆二色谱。表明双酚胺酸既不嵌入DNA分子也不形成加成物,它的致畸作用很可能是螫合作用而导致母鼠缺乏某些金属离子所致,而并不影响DNA或染色体。更多还原

【Abstract】 Catecholamic acid(catechol-3, 6-bis(methyleneimino diacetic acid), CBMIDA), a chelating agent, was shown to be effective in treatment of uranium intoxication. But it is possessed of obviously teratogenic and embryotoxic effects on wistar rat embryos.CBMIDA sc 100, 200, .500 mg/kg considerably increased the number of resorbed and teratic fetuses.However, it did not induce chromosome aberrations in CHL cells at the concentration of IC50. CBMIDA did not increase the frequency of micronucleated PCE in ICR mouse bone marrow. Further studies on effect of CBMIDA on DNA conformation indicate that the CD spectra of calf thymus DNA were not altered by CBMIDA at 50 ug/ml,. It is thought that teratogenesis caused by CBMIDA results not from the effects on DNA or chromosome, but more likely, from chelating which leads to a shortage of metal elements in the body.更多还原