【摘要】 <正> 如何获取高纯度病毒颗粒作为进一步研究的实验材料,是病毒分子生物学研究中一个需要解决的方法学问题.目前常用的病毒分离方法,如蔗糖梯度离心法和氯化铯平衡密度离心法存在一些不足.硅溶胶作为密度梯度离心介质早已成功地用于细胞及亚细胞结构的分离.70年代末期,新型的经PVF(聚乙烯吡咯烷酮)修饰的硅溶胶-Percoll问世.本文将Percoll梯度离心法用于脊髓灰质炎病毒(以更多还原

【Abstract】 Centrifugation in Percoll isopycnic gradients is reported as effective and simple method for separation and purification of polioviruses. By this method, not only may one separate poliovirus from simian virus 40 (SV40), but also may separate empty virions from solid virions of poliovirus. The bouyant density in Percoll of solid virion of poliovirus, empty virion of poliovirus and SV40 are 1.084, 1.051 and 1.13, respectively. The recovery rate of virus is about 65%.更多还原