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滤纸血和血清快速ELISA检测囊虫病抗体的比较研究
Comparative Study on the Detection of theAntibody in Patients with Cysticercosis using Blood-absorbed Filter Paper and Serum by Fast ELISA
【摘要】 本文使用滤纸血和血清同步进行快速ELISA试验检测囊虫病抗体的效果作了比较研究。滤纸血和血清的阳性检出率分别是78%、75%。滤纸血和血清的几何平均滴度分别是45和716。选择阳性的血清和滤纸血各5份,在不同时间内进行4次重复试验,血清的OD值变异系数为2~8%,滤纸血为4~12%。滤纸血于4℃冰箱内至少可保存4周,不影响阳性结果的判定。表明滤纸血快速ELISA是一种简便、快速的方法,在囊虫病血清流行病学调查中有其实用价值。
【Abstract】 In this paper comparative study of the detective effect of the antibody in patients with cysticercosis uning blood-absorbed filter paper and serum was carried out. The positive rate of the eluate from the blood-absorbed! filter paper and the serum was 78% and 75 % respectively, they had no obvious difference. The geometric average litre from the eluate of the blood-absorbed filter paper and the serum was 45 and 716 respectively. In different times,the repeated experiments were made four times using 5 positive sera and 5 positive blood-absorbed filter papers. The coefficient of variation of absorbance value from the sera and blood-absorbed filter papers was 2-8% and 4-12% respectively. When the blood-absorbed-filter papers were kept at 4℃ in a refrigerator,the positive reaction was not influenced for at least 4 weeks. Therefore,Fast EL1SA of the blood-absorbed filter paper is a simple and rapid method,it is of practical value in investigating serological epidemiology of cysticercosis.
- 【文献出处】 中国人兽共患病杂志 ,Chinese Journal of Zoonoses , 编辑部邮箱 ,1990年04期
- 【被引频次】3
- 【下载频次】27