【摘要】 从患生殖器官病的病鸭生殖道和肠道中分离大肠杆菌530株,在用TC、SD、CM、KM、GM、SM、AP、Fu、Rif等9种药物进行细菌药敏试验的基础上,进行了R质粒接合传递试验和质粒DNA电泳分析。试验结果表明:大肠杆菌耐药率为59.4％,其中多重耐药菌株占56.5％,细菌对TC抗性最高（54.0％）;耐药菌的R质粒接合传递率为42.6％,对AP抗性的菌株,其R质粒传递率达75.0％。本文还对抗菌药物在该菌R质粒形成与发展中起的作用、鸭生殖道致病性大肠杆菌的可能起源、K₉₉抗原基因与R质粒的连锁,以及细菌抗TC、AP、SD、CM、SM等几种药物基因的连锁遗传等问题作了初步探讨。更多还原

【Abstract】 A total of 530 strains of E. coli isolated from edeitis cases of ducks were tested for drug resistance and transference of R plasmids. Agarose gel electrophoresis of R plasmids were applicated as well. With reference to nine drugs,i. e. TC,CM,SM,SD,KM,GM,AP,FU,RIF,59. 4% of 530 strains were drug-resistant, and among those resistant to the aforementioned nine drugs, 56. 5% were multiple-resistant. TC-resistant strains were isolated most frequency (54. 0%). the R plasmids were demonstrated at a frequency of 42. 6% among these resistant strains. Strains earring AP-resistant,75.0% respectively, were found to carry R plasmid. Several questions,such as the influence of antibiotic on origin and increase of R plasmids,the origin of E. coli of genitals,the linkage of genes for K99 and R plasmids,the linking heredity of R plasmids which regulate the TC-, AP- ,SD- ,CM- ,and SM-resistance, also be discussed in this paper.更多还原