【摘要】 白三烯B₄（LTB₄）系花生四烯酸的5-脂氧酶代谢产物,是炎症反应中的重要介质。本文建立了测定白细胞及兔全血来源白三烯B₄的反相高效液相色谱法, 研究了样品介质的pH、甲醇浓度及血浆蛋白等对生物样品中LTB₄固相萃取的影响,建立了简便实用的萃取和测定方法更多还原

【Abstract】 Leukotriene B^, a metabolite of arachidonic acid via 5-lipoxygenase, is a potent mediator in inflammatory reactions. The RP-HPLC was ernptoyed for measuring L-TB4 generated from rabbit whole blood and polymorphoneuclear leukocytes. We evaluated ,the effects of inethanol concentration, PH and plasma protein on the solid-phase extraction of LTB4 ia biological samples. Simple and practical techniques were developed.更多还原