【摘要】 本文首次报道了根据噬菌体XCP₁的特性以及寄主溃疡病细菌吸中和噬苗体增殖的原理,采用双层琼脂法测定游离噬体的数量变化来检测柑桔溃疡病无症材料,经过四川省12个县、市80个送检柑桔标样的噬菌体吸附或增殖检验,证实它是一种特异性强、灵敏度高、快速准确的先进检测技术。更多还原

【Abstract】 The detecting technique with the bacteriophage XCP₁ of citrus bacterial canker disease in the nonsymptomatic tissues is reported first in this paper.On the basis of the principle of bacteriophage absorption and multiplication, the double-layer agar method was used for determinating the average number of the free bacteriophage plaques to detect the nonsymptomatic citrus tissues, The sensitivity of detection is 10^3-4 CFU/ml and 12-15 hours are required for all the procedures. The determination standard of the absorption detection is P∠ P（ck）-3SD called positive reaction "+", and P≥P_ck-3SD called negative reaction"-" . The determination standard of the multiplication detection is ⅡP>ⅠP+3SD and ⅡP>ⅡP_ck +3SD called positive reaction "+", and ⅡP>ⅠP+3SD and ⅡP≤ⅡP_cK +3SD called negative reaction "-".The 80 samples from 12 counties and cities of Sichuan province were detected with the bacteriophage absorption and multiplication detection techniques, which proved that the techniques were of high sensitivity, specificity, accuracy and rapidity.更多还原