【摘要】 本实验表明,人rIL-4可以促进SAC活化的人扁桃腺B细胞的增殖,且预活化24小时和72小时的B细胞均对IL-4有相似程度的增殖,只是24小时活化的B细胞有较高的本底应答.然而rIL-4支持活化后B细胞增殖的时间相当短,在最初的24小时培育中,B细胞对IL-4有良好的增殖应答,培育在72小时时,B细胞的增殖应答则显著降低。同时表明,以SAC—B细胞测定rIL-4活性的适宜方法是二步法,第一步培育72小时,第二步与rIL-4共育24小时。不溶性Anti-μ活化的B细胞对SAC的增殖应答较SAC活化者为高。无论SAC或Anti-μ活化的B细胞对rIL-2和rIL-4均应答良好,故不能区分在含有此两种因子的天然样品中各因子的单独活性。更多还原

【Abstract】 The responsiveness of SAC-activated human B cells to human rIL-4 remains controversial. We have proved that rIL-4 definitely supports the proliferation of SAC-activated human tonsillar B cells. B cells preactivated with SAC for 24 hr or 72 hr exhibited the same magnitude of response to rIL-4. However, the 24 hr SAC-B cells showed substantially higher DNA synthesis without supply of rIL-4 than did of 72 hr SAC-B cells. The time that rIL-4 promoted proliferation of B cells is short. The growth factor activity of IL-4 for B cells is strongly unraveled in the first 24 hr cultivation and then, becoming weaker with prolonging of culture period. Two-step assay with 24 hr incubation for each step is introduced for the assessment of rIL-4 activity by SAC-B cells. Anti-μ beads activated B cells showed better response to rIL-4 in comparison with SAC-B cells. B cells activated by either anti-μ or SAC responded well to rIL-2 or rIL-4.更多还原