【摘要】 采用聚丙烯酰胺凝胶等电聚焦电泳法对上海地区临床常见对氨苄青霉素耐药革兰氏阴性杆菌(绿脓杆菌同时对羧苄青霉素耐药)中β-内酰胺酶的研究结果。用Nitrocefin法对150株革兰氏阴性杆菌进行酶定性检测,阳性率为88.7％(133/150)。对氨苄青霉素耐药率为67.3％(101/150)。33株对氨苄青霉素或羧苄青霉素敏感产酶株中的31株只产染色体介导的酶。90株耐药产酶株中只产染色体酶的占10％(9株),产质粒介导的酶占90％(81株),其中又以TEM-1酶的分布最广,存在于8种细菌中,其发生率为44.4％(40/90),其次为PSE-1酶,分布于7种细菌中,发生率为15.6％。 TEM-2、OXA-1和PSE-3酶分别为14.4％、12.2％和1.1％,而OXA-3和SHV-1酶较为少见,OXA-2酶则未检测到。更多还原

【Abstract】 β-lactamases were detected by means of nitrocefin plate method in 150 strains 17 species of Gram-negative bacilli, collected from Shanghai district. The enzyme positive rate was 88.7%. The ampicillin-resistant rate(Pseudomonas aeruginosa resistant to carbenicillin)was 67.3%.The types and distribution of β-lactamases in 123 enzyme producing isolates were determided by analytical isoelectric focusing.With 2 exceptions, sensitive enzyme producing isolates produced only chrompsoma-lly determined β-lactamases. Of the 90 drug resistant enzyme producing isolates, 10% only produced chromosomally determined β-lactamases,90%produced Plasmid determined β-lactamases. The most frequently identified type was TEM-1, present in 8 species of bacteria or in 44.4% of the 90 β-lacamases producing and drug resistant strains.Next is PSE-1, occurring in 7 species, with an incidence of 15.6%, TEM-2.OXA-1 and PSE--3 followed behind, occurring in 14.4%, 12.2% and 11.1% of the strains respectively. OXA3 and SHV-1 were identified occasionly. OXA-2 had not been identified.更多还原