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Dot-ELISA在脑囊虫病诊断中的应用

DOT-ELISA IN THE DIAGNOSIS OF NEUROCYSTICERCOSIS

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【作者】 姜洪杰杨连雪庞苏红孙铁谢淑萍孟家眉温桂芝王国志

【Author】 Jiang Hong-Jie, Yang Lian-Xue, Pang Su-Hong, Sun TieDepartment of Parasitology, Capital Medical CollegeXie Shu-Ping, Meng Jia-Mei Institute of Neurocysticercosis, Beijing Xuanwu HospitalWen Gui-Zhi, Wang Guo-ZhiDepartment of Parasitology, Jiamusi Medical College

【机构】 首都医学院寄生虫学教研室北京宣武医院脑囊虫病研究室佳木斯医学院寄生虫学教研室佳木斯医学院寄生虫学教研室

【摘要】 本文报告用Dot-ELISA法检测脑囊虫病患者的血清和脑脊液的抗体。108例确诊为脑囊虫病患者的血清阳性率为81.6~96.1%,14例确诊为脑囊虫病患者脑脊液的阳性率为85.7~100%。54例正常人血清在1:40稀释度以上,均未出现假阳性;与并殖吸虫病患者及华支睾吸虫病患者血清均未出现交叉反应;与包虫病及脑血管疾病患者血清有一定的交叉反应。

【Abstract】 The doteRzyme-linked immunosorbent assa was used to detect Cysticercus cellulosae antibodies in sera of patients with neurocysticercosis. Among 108 confirmed cases of neurocysticercosis 81.6-96.1% showed positive reactions. Two out of 54 normal control sera reacted at a serum dilution of 1:20, but none at a 1:40 (range 40-640). No cross reactions were observed with sera from cases of paragonimiasis and clonorchiasis, but it did occur to some extent with sera from cases of echinococcosis and cerebrovascular diseases. The results indicated that the dot-ELISA was sensitive, specific and economic for the diagnosis of neurocysticercosis.

【关键词】 Dot-ELISA脑囊虫病诊断
【Key words】 Dot-ELISAneurocysticercesisdiagnosis
  • 【文献出处】 寄生虫学与寄生虫病杂志 ,Chinese Journal of Parasitology and Parasitic Diseases , 编辑部邮箱 ,1990年03期
  • 【被引频次】4
  • 【下载频次】17
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