【摘要】 用低温包埋剂Lowicry1 K4M在-30℃下包埋杜氏利什曼原虫前鞭毛体,切片后在电镜下观察到前鞭毛体结构保存较好。用单克隆抗体2H₆-E₅和1H₇-C₂-E₇分别对它们所识别的杜氏利什曼原虫前鞭毛体抗原进行胶体金标记定位,结果发现保护性抗体2H₆-E₃识别的抗原主要分布于前鞭毛体膜外侧面,为进一步研究该抗原的组成、性质和功能建立了一定基础。同时发现1H₇-C₂-E₇识别的抗原主要分布于前鞭毛体膜内侧面,膜外侧面也有分布。更多还原

【Abstract】 By using Lowicryl K4M, a kind of low temperature embedding resin, to embed promastigotes of Leishmania donovani at - 30℃, the protozoal structure was well preserved when visualized by electron microscope.We have determined the distribution of the promastigote antigen of human and canine isolates of Leishmania donovani from Sichuan using two monoclonal antibodies combined with biotinylated goat antimouse IgG and streptavidin/colloidal gold conjugates. The result showed that the antigen recognized by the protective monoclonal antibody 2H6-E3 was mainly distributed in the outer side of promastigote membrane of Leishma-nia donovani Sichuan canine isolate. According to the high density of localized gold particles this antigen might be abundant in the promastigote membrane (Fig.1). This result facilitates further research on the component, property and function of this antigen.Monoclonal antibody 1H7-C2-E7 recognized the promastigote antigen of both human and canine isolates of Leishmania donovani from Sichuan, suggesting that these two isolates might share a common antigen, and 1H7-C2-E7 antibody might be used to diagnose the leishmaniasis caused by these two isolates (Figs.2-3).更多还原