【摘要】 将杜氏利什曼四川分离株 kDNA 微环连接于质粒 pUC18的 BamHI 位点上转化入大肠杆菌DH52,通过重组质粒的分子量及 BamHI 消化产物的分子量测定,重组质粒与利什曼原虫前鞭毛体及kDNA 杂交,鉴定出该 kDNA 微环与 pUC18重组后获得克隆。从而即可迅速大量地产生均一的微环分子,作为鉴定利什曼原虫的探针,还可作研究环境诱变剂的靶分子。更多还原

【Abstract】 The minicircles of Leishmania donovani Sichuan human and canine isolate.whieh was linearized by BamH I,were inserted at the restriction endonuclease BamH I site of bacteria plasmid pUC18,and the ligated DNA was transformed into E.coli DH5a cells.The recombinant DNA which molecular weight is equal to the sum of molccular weight of pUC 18 and minicircle was obtained,and when the recombinant DNA is digested by BamH I,the molecules of pUC 18 and minieirele can produced.The rece- mbinant DNA can.hybridized with uncloned kDNA,and isolates of Leishmania donovani but cannot do with human genomic DNA,Romanomermis genomic DNA,leptospiraI genomic DNA,and L.donovani genomic DNA.These results showed that the minicir- cles of L.donovani have been eloned.The cloned kDNA minicircle is useful in producing large number of homogeneous minicirclcs and can be used for identification of Leishmania and as the target molecule of enviromental mutagen.更多还原