【摘要】 本文采用SCE和染色体G显带技术,对二亚硝基哌嗪体外转化的人胚鼻咽上皮细胞进行了细胞遗传学的观察,结果显示:该转化细胞的染色体数波动在62～77之间,众数为74;非整倍体核型,并出现结构异常的"标记"染色体18条(M₁～M₁₈);SCE频率为9.76±0.51,比正常对照细胞明显增高。文章还对染色体异常及SCE在细胞体外恶性转化过程中的可能意义作了初步分析。更多还原

【Abstract】 An analysis of chromosomes in DNP-transformed human embryonic nasopharyngeal epithelial (HENPE) cells was performed by the G-banding and SCE technique. Data demonstrated that the chromosomal structure and number were aberrant. The number of chromosome increased from 46 to more than 70, fluctuating between 62 to 77, 18 marker chromosomes were encountered frequen tly.In comparison with the chromosomal aberration of nasopharyngeal carcinoma (NPC) solid tumors, cell lines and partial transformed cells reported in the literature, no similarity was found. In addition, SCE freqnency (9.76±0.51) was significantly higher than that of normal HENPE cells (5.20±0.79) but far less than that of NPC cell line. These changes may be related to DNA damages induced by DNP.更多还原