【摘要】 本文采用非选择性培养基对22名健康青少年的唾液、沟裂菌斑、龈上菌斑及龈下菌斑中的需氧菌、兼性厌氧菌及专性厌氧菌进行了分离培养,并计算其在不同标本中占可培养菌的百分比及检出率。结果共分离到包括18个菌属的35种细菌。其中,链球菌、放线菌、奈瑟氏球菌、二氧化碳噬纤维菌、类杆菌、梭杆菌,奴卡氏菌及棒状杆菌在口腔4个部位的检出率及所占比例均较高,是健康青少年口腔中的优势菌群.通过比较还发现,其中一些菌在口腔4个部位的分布存在一定差异.本文还采用刚果红负性染色涂片法,镜下观察龈上、龈下菌斑中的螺旋体,并计算其相对比例.结果龈下菌斑中螺旋体的相对比例明显高于龈上菌斑.更多还原

【Abstract】 This study investigated the oral normal flora of 22 healthy teenagers.Two Kinds of non-selective culture media were employod. The specimens were taken from saliva,fiss- ure plaque,supragingival plaque and subgin- gival plaque for each subject.we obtained 35 species belonging to 18 genera.Of these bacteria, Streptococcus,Actinomyces,Neisseria,Bacte- roides,Fusobacterium,Capnocytophaga,No- cardia and Corynebacterium,had higher freque- ncies of isolation and proportions at each site,They were considered predominent oral flora. There were differences in the distribution of some predominent flora statistically. These results might signify differences in the properties of different micro-ecosystem or micro-communities. Congo Red negatively-stained smear was also employed to examine the spirochates in subgingival and supragingival plaque micro- scopically.The % of spirochates in subgingival plaque was higher than that in supragingival plaque.更多还原