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马兜铃中马兜铃酸A的HPLC测定

Quantitative Determination of Aristolochic Acid A in the Fruits of Dutchmanspipe (Aristolochia) by HPLC

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【作者】 刘燕; 王宝琴;

【Author】 Liu Yan, National Institute for the Control of Pharmaceutical and Biological Products, Beijing

【机构】 中国药品生物制品检定所;

【摘要】 建立了马兜铃中马兜铃酸A的HPLC测定方法,并对不同产地的马兜铃进行了含量测定。HPLC条件:层析柱YWG-C18 10μm,流动相:甲醇-水-乙酸(70:29:1),流速1ml/min,检测波长315nm。回收率可达97%以上。

【Abstract】 A simple, sensitive method for the HPLC determination of Aristolochic acid A has been established, and the plant samples obtained from different localities were determinated. The optimum HPLC conditions were as follows: column: YWG-C18.10μm; mobile phase: Methanol-Water-Acetic acid (70:29:1); flow rate: 1 ml/min; detection wavelength 315nm. The recovery was above 97%.

【关键词】 马兜铃; 马兜铃酸A; HPLC测定;
  • 【文献出处】 中草药 ,Chinese Traditional and Herbal Drugs , 编辑部邮箱 ,1990年06期
  • 【分类号】R284;O657.72
  • 【被引频次】6
  • 【下载频次】33
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