【摘要】 <正> 许多生化技术和医学诊断方法需要高纯度的细胞亚群样品。激光流式细胞分选术是一种新的物理分离方法,它根据单细胞水平上检测到的多种物化特性进行分选,具有高纯度的特点。这一技术已影响着生物医学的诸多领域。分选原理激光流式细胞分选的主要原理是:单分散的染色细胞在高速流动状态下依次恒速通过激光激发区,产生荧光或散射光,这些光信号反映了细胞的 DNA、RNA 和体积等参数,经变更多还原

【Abstract】 This article presents the design of a laser flow cell sorter.With China-made com-ponents,the instrument can sort interested cell population.With taper model of the flow cham-ber,the cell jet is broken off completely under 11m/s jet velocity,43 kHz ultrasonic vibrationfrequency and 0.47w driving power.The experiment shows,with 125 v charging pulse and ±1.7 kv/cm high-voltage electristatic field,the charged cells can deflected 15 mm from main st-ream after passing about 160 mm in length.Five droplets are charged at the same time in order tosort one interested cell with the duration of charging pulse 122μs.The sorting purity is over80% and the sorting rate is over 1000 cell/s.更多还原