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长毛对虾必需氨基酸的研究

Essential Ami no Acids of the Prawn Penaeus penicillatus

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【作者】 沈国英王平萍苏永全

【Author】 Shen Guoying Wang Pingping Su Yongquan Department of Oceanography

【机构】 厦门大学海洋学系厦门大学海洋学系

【摘要】 分析了不同体长组的长毛对虾生化组成和必需氨基酸(EAA)的可能种类。结果表明:(1)长毛对虾的最主要生化物质蛋白质的含量似有随体长增长(40 mm至 104 mm)而升高。但糖类含量较稳定;(2)注射3H-葡萄糖后测得 Glu,Asp,Ser,Pro,Gly,(Cys)2和 Ala等带有较强的放射性,长毛对虾可能从葡萄糖合成这些氨基酸。而极少或甚至没有3H 参入到Met,Thr,He,Leu,Val,Arg,His,Lys,Phe,Tyr和Trp中。这些可能是长毛对虾的EAA。

【Abstract】 The biochemical compositions of different size P.penicillalusdetermined in this paper show that contents of animal proteins change with the body sizes and vary slightly in glycogen. Seven amino acids,i.e. glutamic acid, alanine, aspartic acid, cystjnc, glycine, proline and serinc, could be synthesied de novoby the prawn after investigating by a radio-isotopic method using (1-3H)-glucose. The constituent amino acids of the protein fractions were isolated by paper chromatography on paper Xinhua No I, with no tryphtophan examined, and then measured using Tri-carb 4 300 Liquid Scintillation Counter (Packard Iustr. Comp.USA). It shows that methionine, valine phcnylalanine, arginine, threnine, histidinc, lysine, isoleueine, leucine and tyrosine have been, little or even not, incorporated radioactivity from 3H-glucose. These amino acids are considcred to be prabably essential ones for this animal.lt also reveals that the smaller prawn synthesized more radioactivities in protein from glucose than that of bigger one.

【关键词】 长毛对虾必需氨基酸
【Key words】 Penaeus penicillalusEssential amino acid
【基金】 福建省水产厅资助的对虾营养研究课题
  • 【文献出处】 厦门大学学报(自然科学版) ,Journal of Xiamen University(Natural Science) , 编辑部邮箱 ,1990年03期
  • 【被引频次】7
  • 【下载频次】36
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