【摘要】 用40mmol/L NaF 对金鱼黄素细胞作体外处理,发现 F~-可以增强细胞内蛋白质的磷酸化作用,特别是能够增强其色素细胞器的一种主要成分——分子量为57 000的蛋白质(简称 p57)磷酸化作用2.2～14倍。但是,F~-的这种刺激效应在缺乏 cAMP(环腺苷酸)的情况下大于有外源性 cAMP 的情况,且可被依赖于 cAMP 的蛋白激酶抑制剂所削弱。更多还原

【Abstract】 Previous studies had shown that a protein p57(Mr 57 000)is a major com- ponent of pigmet organells of goldfish xanthophores.p57 phosphorylation plays a key role in regulation the intracellular movement of the organelles.Here we report that fluoride stimulated increase in the phosphorylation of P57 2.2—14 fold either in the presence of cAMP,protin kinase inhibitor(pKI) phosphodiasterase or in the absence of them(separatlly or combined)in vitro. The increase is more in the absence of cAMP than in the presence of cAMP, and the effect of fluoride is reduced by adding PKI.Moreover,fluoride stimulated increase in the phosphorylation of other proteins of xanthophore更多还原