【摘要】 用亚硝基胍（NTG）和紫外线（UV）诱变处理出发菌株T_6-13,定向筛选α-氨基β-羟基戊酸（AHV）和S-（2-氯基乙基）-L-半胱氨酸（AEC）抗性突变株和蛋氨酸缺陷型,得一苏氨酸产生菌M_8-31（AHV^rAEC^rMet^-）,可积累L-苏氨酸6.7mg/ml。再以M_8-31为出发菌株,进一步提高抗性和筛选α-氨基-4-乙硫丁酸（Eth）敏感突变株,获得一苏氨酸高产菌株ME7（AHV^rAEC^rMet^-Eth^s）,在含有葡萄糖10%的培养基中发酵48h可积累L-苏氨酸17.5mg/ml。更多还原

【Abstract】 Brevibacterium flavum Te_6-13 a glutamate producer, was treated with N-methyl-N’-nitro-N-nitrosoguanidine（NTG） and ultra-violet（UV） rays. As a result, a meth-ionine auxotrophic mutant M_8-31 was derived which was resistant to both a-amino-β-h drox valeric acid（AHV） and S-（2-aminoeth 1）-L-cysteine （AEC）. It accumu-lated 6.7mg/ml of L-threonine. With M_8-31, as a starting strain, after receiving mi-xture mutational treatment with UV+LiC1 and NTG,a 2-amino-d-eth Isulphobutanoicacid（Eth） sensitive mutant ME₇ was obtained. It produced 17.5mg/ml of L-threoninein a medium containing 10% glucose in 48 hours at 33℃.更多还原