【摘要】 本实验以赭曲霉F₄₄₉为出发菌株,发酵产生氨基酰化酶,其野生型菌株产酶单位为12.2μ/ml发酵液。我们首先以紫外线诱变处理2min,得到其变异株F₉,产酶16μ/ml发酵液,然后对F₉菌株的原生质体进行亚硝基胍诱变处理15min,得F₂₈菌株,产酶24.7μ/ml发酵液,将F₂₈菌株在优化培养基中发酵培养,其产酰化酶单位提高到32.4μ/ml发酵液,与此同时,提取得到粗品酰化酶,其酶活为15,000μ/g。更多还原

【Abstract】 Aspergillus ochraceus F₄₄₉ was used as the starting strain in the experiment.Through fermentation, it produced aminoacylase 12.2u/mi. When A. ochraceusF₄₄₉ had received UV radiation for 2 minutes, mutant F₈ was obtained, whichproduced aminoacylase 16u/ml. When protoplast of mutant F₉ was treated for 15min. with NTG（N-Methyl-N’-nitro-N-nitrosoguanidine）. Mutant F₂₈ was obtai-ned, which produced aminoacylase 24.1u/mi. When mutant F₂₈ had been culturedin the finest medium, the yield of aminoacylase was 32.4 u/mi. N-acethyl-DL-phenyalanate was used as substrate, 25.9 percent of which was converted into L-phenylalanate by aminoacylase. In the meantime, raw aminoacylase was extracted,its activity being 15000u/g.更多还原