【摘要】 本文报道了基因探针在大肠杆菌中的扩增与提纯方法。含基因探针的质粒用氯化钙方法转染大肠杆菌,被转染的细菌与质粒在培养液中同步扩增,然后用碱裂解法抽提扩增后的质粒。结果表明:(1)质粒的转化效率与其浓度有关;(2)经转染、扩增与抽提后,获得了大量的纯化质粒DNA;(3)纯化后的基因探针能满意地用于DNA 标记及分子杂交。更多还原

【Abstract】 A method for amplification and purification of gene prode in Escherichia coli(E.coli)is reported in this paper.Plasmids containing gene probe were transfectedinto E.coli by treating bacteria cells with calcium chloride.The transfected bacteriawere cultured to allow plasmid amplification.Then the amplified plasmids wereextracted from E.coli by alkline lysis procedure.The results are as follows:(1)Theefficiency of transformation of plasmid is related to its concentration.(2)Aftertransformation,amplification and extraction,a large number of plasmids DNA areacquired.(3)Purified gene probe can be satisfingly used in DNA labelling andmolecular hybridization.更多还原