【摘要】 本文介绍了5例ANLL 患者骨髓L-CFU 体外完全无血清培养方法。培养体系中必须加入某些物质,以取代小牛血清。结果表明,加入2×10^-4mol/LBSA 第5部份、400μg/ml 饱和铁的人运铁蛋白、1U/ml 胰岛素及20μg/ml 胆固醇,对L-CFU 生长十分重要。加入1×10^-4mol/L2-ME 可使L-CFU 集落增大。全部实验均用HTB9-CM 作为刺激因子。本方法为研究人类造血生长因子对L-CFU 增殖调控以及将来L-CFU 培养的标准化,提供了先决条件。.i.———.———.一…..、.更多还原

【Abstract】 A method using completely serum-free culture system for L-CFU assay in vitroon 5 cases of acute non-lymphocytic leukemia is reported.Some agents were testedfor the requirement of L-CFU assay in order to replace the bovine serum using in theconventional culture medium.The result shows that fraction V of bovine serum albu-min (2×10^-4mol/L),iron saturated human transferrin（400μg/ml）,insulin （1U/ml）,andcholesterol（20μg/ml）are the basic components for ANLL L-CFU growth.Increasein colony size can be obtained by adding 2-Mercaptoethanol.HTB9-CM as growth sti-mulating factor was used in all experiments.This method may be critical for the studyof L-CFU proliferation regulation by human hemopoietic growth factor and the stan-dardization of L-CFU assay in the future.更多还原