【摘要】 本文对纯化了的猪肝DFPase^[**]进行了一系列结构和性质的测定。用重复中毒的方法证明了猪肝中低分子量二异丙基氟磷酸酯（DFP）水解酶（DFPase）是属于催化酶。分析了DFPase的氨基酸组成并求出其分子量为17900。该酶的km值为6.7×10^-4mol/L。催化水解DFP的最适pH为7.5～7.7,反应最佳温度为70℃。根据催化水解DFP、Soman的活力以及Mn²⁺对此酶的影响证明该酶属于Mazur type DFPase^[1]。更多还原

【Abstract】 This paper is about the studies of the structure and some properties of pure DFPase from pig liver. As shown by means of repeated hydrologtic poisoning with DFP as substrate, that the small DFPase from pig liver was a catalytic enzyme. The molecular weight of DFPase was 17 900 by the analysis of amino acids after acid-hydrolysis. Using DFP as substrate,the Km value was 6.7×10-4 mol/L,the optimum pH was 7.5-7.7 and the optimum temperature was 70℃. The DFPase hydrolyzed Soman faster than DFP, and it was activited by Mn2+. These results suggested that the small DEFase was of Mazur type.更多还原