【摘要】 作者通过实验证明“紫外吸收法测定核酸(RNA)含量”的实验方法中,所用核酸沉淀剂钼酸铵—过氯酸试剂有较大的260nm吸收值。其用量不足或过剩都会致使RNA定量测定结果明显偏低。通过对几种核酸沉淀试剂的比较,发现用酸性乙醇代替钼酸铵——过氯酸试剂增加测定结果的可靠性。如待测RNA样品中存在蛋白质,则测定结果偏高。用氯仿去除蛋白,可以大大减少干扰。更多还原

【Abstract】 It was proved that nucleic acid precipitant molybdate ammonium--perchloric acid has a larger absorption at 260nm in the method of "Quantitative determina- tion of RNA by UV absorption spectrometry" through out experiment. The result would decrease obviously if the amount of the precipitant was insufficient or exc- essive We found that the use of acidic ethanol instead of molypdate ammonium made the result much more reliable by comparing several nucleic acid precipitants. The presence of protein in the sample would make the result higher, The removal of protein with chloroform could clecrease the interference greatly.更多还原