【摘要】 本文采用RP-HPLC和二极管阵列检测器建立了小蔓和大蔓长春花中长春胺的快速定性和定量测定方法,利用HPLC保留时间和二级管阵列检测器给出的紫外光谱进行定性,并用半制备HPLC方法将长春胺收集纯化后用质谱和红外光谱加以确证。小蔓长春花中的长春胺含量可直接从乙醇萃取液中测定,含量超过千分之五。大蔓长春花中长春胺含量低于十万分之一、须经一次制备分离后才可测定。更多还原

【Abstract】 A rapid method for the separation and determination of vineamine in Vinca minor and Vinca major by reversed-phase high performance liquid ehromatography (Rp-HpLC) using a photodiode array detector has been developed on μBondapak C18 column with methanol and phosphate buffer solution as the mobile phase.In order to confirm the component separated from the total alkaloid,the peak coataining vincamine was collected by a semi-preparative column and then corroborated by MS and IR.The content of vincamine was determined directly from the ethanol extract of the plant Vinca minor.The concentration of vincamine in Vinca minor planted in China is about 0.5%.Owing to the very low content (less than 0.001%) of vincamine in Vinca major,the vincamine was preconcentrated with a semi-preparative columu and then determined by the same procedure as used for Vinca minor.更多还原