【摘要】 本文结合免疫金银染色(IGSS)法和图像定量分析,观察了幼年(2个月)、中年(10个月)和老年(24个月)Wistar大鼠脑于中缝核群5-羟色胺样(5-HT-LI)神经元的衰老变化。结果表明:(1)在衰老进程中,5-HT-LI神经元仅于老年组的中缝桥核和嘴侧线形核有显著减少(p<0.01);(2)从幼年到中年,5-HT-LI神经元截面积几乎增大一倍,但到老年无显著性变化;(3)5-HT-LI神经元的灰度值在整个衰老进程中呈降低趋势,提示神经元的5-羟色胺的含量和活性随增龄而升高;(4)5-HT-LI神经元形态的衰老变化表现为胞体萎缩,边缘皱折而呈不规则状,突起缩短锐化。更多还原

【Abstract】 Combining the immunogold-silver staining method and the image quantitative analysis, aging changes in serotonin-like (5-HTL) neurons of the raphe nuclei of the brainstem were observed in young (2 months), adult (10 months) and old (24 months) Wistar rats. The results were as follows: Ⅰ. number of 5-HTL neurons decreased significantly in the nucleus raphe pontis and Nucleus linearis oralis of the old rat only (P<0.01). Ⅱ. Size of the neurons almost doubled from the young to the adult, how ever, there was no marked changes in the old rat. Ⅲ. Gray value of the neurous declined throughout the aging period. This suggested that the content and activity of serotonin in the raphe nuclei increased with aging. Ⅳ. Morphological changes of the neurons included cell body shrinkage, folding of the edges and shortening and sharpening of the processes.更多还原