【摘要】 本文应用免疫细胞化学方法对金黄地鼠17区、18a区和17/18a交界区的γ-氨基丁酸(GABA)阳性神经元的密度和胞体面积进行了研究。结果表明此三区的各层均有GABA阳性神经元分布,细胞形态为圆形、卵圆形、梭形、多极和双极形。密度以17区为最高,18a区次之,交界区最低(Ⅰ层除外)。阳性神经元在各区Ⅰ层高达75％,其它层占各层神经元总数的8％-14％。胞体面积的测量与分析表明阳性神经元胞体面积变化颇大,介于40-240μm~2的范围内。在17区,Ⅰ层和和Ⅳ层的阳性神经元明显小于其它层。在18a区和交界区,除了Ⅰ层和Ⅳ层以外,Ⅱ、Ⅲ层阳性神经元也比较小。17区阳性神经元的平均胞体面积大于18a区和交界区。更多还原

【Abstract】 Physiological studies have shown that inhibitory circuits play an important role in constituting receptive fields of the visual cortex. And GABA-containing neurons are, in most cases, responsible for such inhibitory operations. In the present study, we used a primary antiserum directed against GABA to identify GABA-containing neurons in areas 17, 18a and the 17/18a border region of the adult hamsters and a comparison of laminar distribution and perikaryal surface areas of GABA-immunopositive (GABA-IP) neurons of these areas were conducted.GABA-IP neurons are found throughout all layers of areas 17 and 18a. In layer I, up to 76％ of the neurons ware GABA-immunopositive, and no density difference between areas 17, 18a and the border region was detected. In the remaining layers, the density of GABA-IP neurons in the 17/18a border region was low as compared with the density in the 17 and 18a areas proper.The shape of the GABA-IP neurons were heterogeneous, including fusiform, bipolar, multipolar, round and ovoid, but no GABA-IP pyramidal neurons were observed.The perikaryal surface area of GABA-IP neurons varied widely from 40-240 μm~2. In area 17, layer Ⅰ and Ⅳ GABA-IP cells were significantly smaller than that in other layers, and in area 18a layers Ⅱ/Ⅲ GABA-IP cells were smaller as well. In general, the mean perikaryal surface area of GABA-IP neurons in area 17 was larger than that in area 18a and the border region.更多还原