【摘要】 1987年6月27日至1988年5月11日,在上海市奉贤县进行一次蚊虫的乙型脑炎病毒感染率和分布特点的研究。捕捉的39923只蚊虫中,92％为三带喙库蚊。以蚀斑形成试验分离到103株病毒,经间接免疫荧光试验鉴定为乙型脑炎病毒。三带喙库蚊的乙型脑炎病毒感染率为3.5％;含孕卵蚊的混合标本的乙型脑炎病毒检出率为47.6％,显著(X~2=17.94,P<0.01)高于不含孕卵蚊的混合标本的23.3％;每个混合标本含的有孕卵蚊数愈多,检出率愈高(X~2=24.83,P<0.01)。 阳性混合标本在细胞培养合内的频数分布能符合Poison曲线(P>0.3),反映了带乙型脑炎病毒的蚊虫在自然界蚊群中的分布是随机的。更多还原

【Abstract】 A study on the inoideno and distribution of Japanese encephalitis virus infected mosquitoes among the mosquito population was carried out Shanghai from 27 June, 1987 through 11 may, 1988 in FengXian county.39 923 mosquitoes were collected, most of them (92%) were Oulex tritaenior-hynous. 103 isolates were obtained by a system of plaque assay. These isolates were identified as Japanese encephalitis virus by indirect immunofluorescens assay. The natural infection rate in mosquitoes was estimated as 3.5‰, In pools wibh gravid Oulex tritaeniorhynohus there was a positive rate of 47.6%, as compared to 23.3% for those with deplete mosquitoes (x2 = 17.941, P<0.01). No virus was detected in over winter mosquitoes nor in mosquitoes trapped in late spring of 1988.Distribution of positive pools in cell culture plates was fitted well with the Poisson series. It is suggested that Japanese encephalitis virus infection of mosquitoes in its population is random in nature.更多还原