【摘要】 从感染小鼠腹水瘤病毒(SRSV)的NIH3T3小鼠成纤维细胞培养液中,分离SRSV,并提取病毒核酸(SRSV RNA),通过超速离心和凝胶电泳测定RNA的沉降系数和分子量。SRSV RNA主要由两部分组成:①沉降系数(8)约60～70S(热处理后约35S)及少量18S和28S;②是4～5S。高分子病毒RNA在网织红细胞无细胞系统中刺激放射性氨基酸掺入。对翻译产物分析表明:至少有3个蛋白的分子量相应于病毒蛋白。更多还原

【Abstract】 SES mouse leukemia virus (SESV) was obtained from the culture medium supernatant of infected NIH 3T3 mouse fibroblasts. SESV ENA was prepared from fresh viral pellets. ENAs of various fractions were determined by sedimentation coefficient followed by gel eleotrophoresis. The components of SESV ENA consist of two main fractions, one sedimenting at about 60 -70S (after treatment with heat at about 35S), plus small amounts of ENA sedimenting in the 18S and 28S areas, and the other at 4 -6S. High molecular weight virus ENA stimulates the incorporation of radioactive amino acids into protein in a reticulooyte cell free system. Analysis of the translational products demonstrated the synthesis of at least three proteins corresponding in molecular weight to several authentic viral proteins.更多还原