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用酶标受体法检测牛生长激素的生物活性
ELISA-RECEPTOR ASSAY FOR TESTING THE BIOACTIVITY OF GROWTH HORMONE
【摘要】 <正> 激素的测定对于研究激素有很大的意义。多少年来激素只能借助于生物效应来测活。随着同位素和免疫学方法的发展,放射免疫分析(RIA)技术已成功地应用于许多人体激素的测定,此法
【Abstract】 According to the principle of the emzyme-linked immunoassay(ELISA), a new method, ELISA-receptor assay (ELISA-RA), for testing the bioactivity of growth hormone was dcvised. In this ELISA-RA system, the growth hormone which has bound to its receptors can also bind to its antibodies. This method can test both the immunoreactivity and bioactivity of growth hormone in a quick and easy way. The sensitivity of this method is 0.5μg of growth hormone. In this ease, it is supposed that there probably is a diffcrenee in the localization between biological activity center and immunoreactivity center or there are more than one bioactivity and immunoreactivity centers in the molecule of growth hormone.
【Key words】 Growth hormone; ELISA; receptor; bioassay;
- 【文献出处】 生物工程学报 ,Chinese Journal of Biotechnology , 编辑部邮箱 ,1990年02期
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