【摘要】 本文选用小苍兰茎尖、球茎和不同品种的花蕾为外植体,分别培养在不同的培养基上,取得了下列结果:1.将茎尖培养在 B₅+0.5mg/LBA 的培养基上,不仅会诱导出愈伤组织,而且也能分化出幼苗,幼苗分化率高达85.3%。2.培养在 MS+4mg/LBA 培养基上的球茎,有80%的外植体能诱导出愈伤组织。愈伤组织中有40%能分化出苗。3.16个品种的花蕾,在 MS+2mg/LBA+0.2mg/LNAA 的培养基上能诱导出愈伤组织,出愈范围为36.1～86.7%。当愈伤组织转入 MS+0.2mg/LBA+0.2mg/LBA+0.2～1.0mg/LNAA 的培养基上时,能分化不定芽和不定根,不定芽的分化率为2.3～86.4%。更多还原

【Abstract】 Tme meristem-tip,corm and the flower buds from 16 cultivas of Freesia were used as experimental meterials.The different explants were planted on different medium.The result were obtaincd as follows: 1.The meristem-tip about 0.2-0.5mm in length were cultured on the MS and B5 medium supplemented with 0.1mg/L BA and 0.5mg/L BA.On which the calli were induced,differentiate in frerquency of shooting from the meristem tips directly on the B5+0.5mg/L BA was 85.3%. 3.The inducing frequeney of callus from corms on the 1/2MS+4mg/L BA is 80% from which differentiation frequency of buds from calli reached 40%. 3.The flower buds from 16 cultivas were planted on MS medium supplemen- ted with 2mg/L BA,0.2mg/L NAA for inducing callus and adventitious buds. The abventitious buds were transfered into MS medium added 0.2mg/L BA, 0.1-0.02mg/L NAA and then the adventitious roots and intact plantlets occured. The results indicated that all of the those meterials planted on MS medium would induce callus and adventitious buds in the range of 36.1-86.7% and 2.3-86.4% repectiuely.更多还原