【摘要】 用固相法合成了一种新的肽类血管紧张素Ⅰ转换酶抑制剂(AI,Pro-Thr-His-Ile-Lys-TrpGly-Asp)和P物质N末端片段(SP3-4,Gly-Phy),并检测了AI和SP3-4对人血浆、脑和肺组织中血管紧张素Ⅰ转换酶(ACE)的抑制作用,结果表明:①合成的AI和SP3-4经HPLC分离鉴定纯度均为>95％,②AI抑制人血浆(IC50 19±4.6/μmol/L)脑(IC50 26±6.3μmol/L)和肺(2例,IC50 119～14.1μmol/L)中ACE活性,SP3-4对人体内ACE活性无抑制。更多还原

【Abstract】 A new angiotensin I converting enzyme inhibitor(AI, Pro-Thr-His-Ile-Lys Trp-Gly-Asp) and a N-terminal fragment of substance P (SP3-4, Gly-Phy) were synthesized by solid phase method. Their capacity to inhibit angiotensin I converting enzyme (ACE) seperated from human blood, brain and lung was tested by biochemical assay. Results showed that 1. All of the synthetic peptides were_assayed and their purity was>95% as established by HPLC analysis. 2. The AI inhibited the ACE from human blood(IC50 19±4.6μmol/L) brain (IC50 26±6.3μmol/L) and lung (2 cases, IC5011.9~14.1μmol/L) but the SP3-4 failed to inhibited the ACE from human being.更多还原