【摘要】 用差速离心和蔗糖垫离心,仿 Soong 和 Milbrath 的方法提纯 SMV,病毒得率分别为24.5,47.1ms/kg 病叶.用作者的方法提纯 SMV 的产量可达199.2mg/kg 病叶,所得病毒粒体具有生物活性,免疫兔子得到的抗血清效价为1/2048(微量沉淀反应).更多还原

【Abstract】 Differential centrifugation and sucrose cushion centrifugation were used to comparethree methods for purification of soybean mosaic virus.The yields were 24.5,47.1,and199.2mg per kg of the infected leaves.The purified virus was infective.Antisera with a titreof 1/2048(microprecipitation test)were prepared by injecting the purified virus intorabits.更多还原