【摘要】 20头母黄牛在发情周期第12天注射孕马血清促性腺激素（PMSG）3000IU,2天后注射15-甲基-前列腺素 F_2α3mg,于发情当天注射兔抗 PMSG 血清2.4ml。应用酶免疫方法检测 PMSG 处理前和处理后笫48,84和240小时母牛血清孕酮含量和 PMSG 的残留量。结果表明:PMSG 在黄牛体内的半衰期长;根据PMSG 处理后第10天的血清孕酮水平可以预测母黄牛的超数排卵效果。更多还原

【Abstract】 Twenty Chinese indigenous breed cattle cows with normal oestrous cycles were treatedwith 3 000 IU of pregnant mare′s serum gonadotrophin （PMSG） on day 12 of the cycle and 3mg of 15-methyl-prostaglandin F_2α on day 14,followed by 2.4 ml of rabbit anti-PMSG se-rum on the day of oestrus.The levels of progesterone and PMSG were detected in serum ofcows at the time of PMSG administration and at hours 48,84 and 240 after PMSG treat-ment by enzymeimmunoassay （EIA）.The results showed that （1） there was a longer half-lifelength for PMSG in cows and （2） the EIA of serum progesterone on day 10 after PMSGtreatment could be applied to estimate the effect of superovulation in cows更多还原