【摘要】 南农CY-1型细胞融合仪的主线路全部由集成电路组成。它能在电融合小室的电极间产生一个交变电场(5kHz～2MHz,0～250V/cm),使动植物细胞排列成串,其输出的直流融合脉冲(脉宽10～150μs,0～300V)能成功地诱导相邻原生质体局部接触区质膜的可逆击穿而形成融合。用该仪器对小麦(T.aestivum)悬浮培养系和叶肉原生质体、微生物原生质体(水稻恶苗病菌)和动物细胞(小鼠骨髓瘤细胞与脾细胞)进行了融合试验.结果表明,在交变电场中,各种细胞成串率均达90%以上;在融合脉冲作用下,小麦原生质体融合率最高可达90%。用荧光双醋酸脂(FDA)测定活力,结果表明:两种小麦原生质体在2000V/cm 脉冲场强作用下,诱导融合后,成活率达80%～90%。更多还原

【Abstract】 The principls and circuit of electrofusion apparatus,model CY-1,are introduced inthis paper.The apparatus is composed of integrate circuits.The animal cells or plantprotoplasts could be aligned in chains in the alternating field (5kHz～2MHz,0～250V/cm) between the electrods of fusion cell.The direct current pulses (duration 10～150μs, 0～300V) could cause reversible membrane breakdown,then the contacting cellsor protoplats fused.Experiments on electrofusions between wheat protoplasts of cell sus-pension and mesophell cells,microbe protoplasts (Gibberella fujikuroi Wr.),or animalcells (myeloma and spleen cells of mouse) have been done using this apparatus.The re-sults showed that over 90% cells alinged in chains in alternating field,and that the per-centage of fused wheat protoplasts was over 90.Viability test (FDA) indicated that 80%～90% of the fused wheat protoplats induced by field strength 2 000V/cm,duration40μs,3 time pulses were alive.更多还原