【摘要】 本文应用气相色谱仪配以氢火焰离子化检测器测定了红细胞、血清、血浆和尿液中的挥发性脂肪酸。选用不锈钢色谱柱(Φ3×2000mm)内装涂有15%聚乙二醇的 Chromosorb WAW DMCS 担体。汽化室、色谱柱、检测器的温度分别为200℃、150℃、200℃。载气为氮气,流速50ml/min。在此条件下,乙酸、丙酸、丁酸、异丁酸、戊酸和异戊酸均得到较好的分离。酸浓度与峰面积呈线性关系。样品回收率在80%以上。重复性实验符合要求。正常状态下,红细胞、血清、血浆及尿液中主要含有乙酸。更多还原

【Abstract】 In this paper,we determined volatile fatty acids in RBC,serum,plasma and urine by gas chromatographywith a flame ionization detector.The column was a stainless steeltube （Φ3×2000mm） packed 15%polythylene glycol 20M on Chromosorb WAW DMCS.The injector,column,and detector temperature wereheld at 200℃,150℃,and 200℃,respectively.The carrier gas（N₂） flow rate was 50ml/min.In theseconditions,acetic acid,propionic acid,isobutyric acid,butyric acid,isovaleric acid and valeric acid were sepa-rated.Acids concentration was linear to peak area.The recoveries from afore said smples were all over 80%.It is satisfactory both in accuracy and reproducibility.There was acetic acid among normal RBC,Serum,plasma and urine.更多还原