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安络小皮伞中水溶性多糖的研究——Ⅰ.甘露聚糖 FP1 的纯化与结构研究
STUDY ON WATER SOLUBLE POLYSACCHARIDE FROM MARASMIUS ANDROSACEUS(L.:FR.)FR.Ⅰ.PURIFICATION AND IDENTIFICATION OF MANNAN FP1 STRUCTURE.
【摘要】 从安络小皮伞水溶性多糖中分离纯化得一甘露聚糖 FP1。分子量约为24万。经红外光谱、+H-NMR 谱和亲和层析指明为β-甘露聚糖。结构分析采用高碘酸氧化、Smith 降解、完全甲基化 GC、GC-MS 与13C-NMR 分析,分子的主链是β-D-(1→6)连接的甘露糖,支链为β-(1→3),β(1→2)甘露糖,分别连接在主链的 O-3和 O-2上。
【Abstract】 A mannan FP1 has been isolated and purified from the water soluble polysaccharide mixture extracted from mycelia of Marasmius androsaceus.The mole- cular weight abount 240000.It is β-D-mannan examined by I.R.and affinity chro- matography.The structure of FP1 has been determinated by periodate oxidation, Smith degradation,methylation,GC,GC-MS etc.The main chain of the molecules was composed of β-D-(1→6) linked mannose,side chain was β-(1→3) and β- (1→2) linked mannose attached to 0-3 and 0-2 of main chain respectively.
【基金】 科学基金
- 【文献出处】 真菌学报 ,Mycosystema , 编辑部邮箱 ,1990年02期
- 【被引频次】28
- 【下载频次】169