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测定RUBISCO羧化反应和加氧反应的方法
A Method for Synchronized Measurement of RUBISCO Carboxylation and Oxygenation
【摘要】 在氧电极反应杯中加入RUDISCO,RuBP,NaHCO2—HaH14CO3,Mg++等,体系中的RUBISCO的羧化反应和加氧反应同时发生,耗氧速率用氧电极测定,羧化速率用液体闪烁仪测定。随着体系CO2浓度的提高,RuBPCase活性提高而RuBPOase活性降低。
【Abstract】 RUBISCO,RuBP,NaHCO3-NaH14CO3,M++,etc were added to a glass reaction vessel(Fig. 2). RUBISCO carboxylation and oxygenation in the system took place at the same time. The rate of O2 consumption was assayed with an O-2-electrode and the rate of carboxylation was assayed with a scintillation counter. The RuBPCase activity was rose whereas the RuBPOase activity declined as the CO2 concentration increased in the system (Fig. 4).
- 【文献出处】 江苏农业学报 ,Jiangsu Journal of Agricultural Sciences , 编辑部邮箱 ,1990年S2期
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