【摘要】 用止血钳夹持加工成楔形的显微手术刀片,在解剖镜下对准胚胎中轴迅速下切,使内细胞团,滋养层和透明带均分为两半。将形态完好的半胚移入受体黄体侧子宫角内,观察妊娠产羔结果。试验共切割15枚囊胚,得到11.5对完好的半胚,切割成功率为76.7％。将其中的10对半胚移植入5头受体母羊,其中2头各移植一对半胚,各获得1对同卵双羔,另一头移植2对半胚,产下1对同卵双羔及1只半胚羔羊。本研究采用简易胚胎分割法获得较高成功率。更多还原

【Abstract】 A simplified method of embryo bisection was established by using the general stereomicroscope, which could be applied to "on-farm" operations. Donors were superovulated and synchronized with recipients. Goat embryos of 6-8 days recovered from donors were quickly bisected into symmetric half embryos with equal inner cellmass, trophoblast and zona pellucida with a surgical microblade held with hemostatic forceps. The embryos which were morphologically good were transferred into the recipient uterus ipsi-lateral to the corpus luteum, and pregnancy and parturition were observed. Of 15 blastocysts, 11. 5(76. 1%) were successfully bisected into good half embryos. Two of five recipients each produced a pair of monozygotic twins. Another recipient, into which two pairs of semi-embryos were transferred, gave birth to a pair of monozygotic twins and a single half-embryo kid. Fairly high success rates of goat embryo bisection were obtained by this method.更多还原