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老化及年青红细胞电介质电泳的研究

Studies on Dielectrophoresis of Aged and Young Erythrocytes

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【作者】 于小波郑静文潘华珍

【Author】 Yu Xiaobo Zheng Jingwen Pan Huazhen (Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing)

【机构】 中国医学科学院基础医学研究所中国医学科学院基础医学研究所

【摘要】 本文介绍我们实验室自己试制的电介质电泳装置及测量方法,并用电介质电泳测定老化及年青红细胞的电介质电泳收集率(DCR)、细胞膜镶嵌蛋白分子水平扩散运动特征频率(Fc)及细胞在交流电场作用下的变形程度。并从测量结果分析老化及年青红细胞变形性及膜导电性、流动性、韧性、脆性等流变学性质。其中细胞变形性测定结果与衍射法进行了比较。

【Abstract】 The dielectrophoretic method and instrument made in our laboratory were introduced in this article. The DCR.Fc and deformation of young and aged ery-throcytes exposed in an AC nonuniform electric field were measured by above instrument and method.The rheololonical properties of young and aged ery-throcytes, including deformation of cell, fluidity of membrane lipid and con-ductibility, tenacity and brittleness of membrane, were analysed from above results. The deformation of cell was also measured by ekiacytometer. The result measured by ekiacytometer was compared with that by dielectrophoresis.

【基金】 国家自然科学基金
  • 【文献出处】 基础医学与临床 ,Basic Medical Sciences and Clinics , 编辑部邮箱 ,1990年01期
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