【摘要】 <正> 紫菜是国内外养殖最多的重要经济海藻之一。1982年唐延林首次用酶法分离出紫菜体细胞和原生质体,并培养获得再生植株,随后在国内外相继开展了紫菜单细胞和原生质体的分离工作。在短短的几年里,紫菜的基础和应用研究均取得明显的进更多还原

【Abstract】 Using an enzyme mixture of 2% sea snail enzymes and 1% cellulase we success fully isolated protoplasts from vegetative cells of Porphyra haitanensis and P. yezoensii, and studied their genetics and breeding techniqus. Many pure lines of P. yezaensis, in which the characteristics of every single blade were essentially identical, were obtained after individual blades of different color and different morphology were isolated and cultured seperately. Low dosages of UV irradiation increased cell division, while higher dosages increased variation rate. When treated with colchicine, the death rate rose and cell division were apparently inhibited as the colchicine concentration increased. Thalli regenerated from the treated groups showed a variety of shapes. Their cells were obviously larger than those from the control group and were irregularly organized. PEG-induced interspecific protoplast fusion was observed and distinguished either by the natural color difference between these two species or by the neutral red labeled P. yezoensis protoplasts. The hybrid cells could be divided and form callus-like cell masses, but could not differentiate into thallus.更多还原