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川白芷中香豆素类成分的反相高效液相色谱分析
REVERSE-PHASE HPLC DETERMINATION OF COUMARINS IN THE TRADITIONAL CHINESE DRUG CHUAN BAIZHI
【摘要】 本文用反相高效液相色谱法分离测定了川白芷的根、茎、叶、尾根及种子中的欧前胡素、异欧前胡素和氧化前胡素等三种有效成分。在键合相0DS柱上,以甲醇一水(70:30)为流动相,紫外检测,样品中各组分分离良好,用微处理机按外标法定量,变异系数≤3.33%,回收率97.91—101.36%。
【Abstract】 A reversed phase H-PLC procedure has been developed for theseparation and determination of oxype-ucedarin(Ⅰ), imperatorin(Ⅱ) and isoi-mperaiorin (Ⅲ) in the root, stem, leat,small root, seed of chuan baizhi simulta-neously, the results were calculated bystandard method on the basia of peakarea. this method is rapid, reproducibleand accurate and has been applied toanalyse the contents of three co-mponents.
【关键词】 白芷;
欧前胡素;
异欧前胡素;
氧化前胡素;
反相高效液相色谱;
【Key words】 Angelica dahurica; Oxypucedarin; Imperatatorin; Isoimperatorin; Reverse-phase HPLC;
【Key words】 Angelica dahurica; Oxypucedarin; Imperatatorin; Isoimperatorin; Reverse-phase HPLC;
- 【文献出处】 华西药学杂志 ,West China Journal of Pharmaceutical Sciences , 编辑部邮箱 ,1990年04期
- 【被引频次】31
- 【下载频次】156