【摘要】 本文应用化学发光免疫测定法,以辣根过氧化物酶（HRP）标记抗体,采用夹心法,用鲁米诺-对碘苯酚-H₂O₂发光体系,测定0.0μU～30μU猪胰岛素,双对数标准曲线的相关系数为0.990,绝对检测限0.05μU,比国产胰岛素免放测定盒灵敏20倍。更多还原

【Abstract】 Chemilum nescent Im-munoassay （CLIA） has both high sensiti-vity of chemiluminescent analysis andspecificity of immunoassay, while it canget rid of the disadvantages of RIA, suchas radioactive hazard and high cost offacilities. In this report, horseradish pe-roxidase （HRP） was used as the label toprepare HRP-Ab conjugate （Ab standingfor anti-porcine insulin guinea pig anti-body） and polystyrene beads were usedto form the solid-phase Ab. Porcine ins-ulin was determined with sandwich pro-cedure and para-iodine phenol added tothe chemiluminescent system of luminol-H₂O₂ （HRP） as a chemiluminescence en-hancer to improve the determination se-nsitivity of HRP. o. 03 μU-30 μU of insu-lin were determined with a limit ofdetection of 0.05 μU, indicating that thismethod is 20 times more sensitive thanChina made RIA kit for insulin.更多还原