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大鼠睡眠的简化多导图描记法的研究

A Simplified Polygraphic Method for Studying Sleep in the Rat

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【作者】 杨俊业; 徐建国; 杨世若;

【Author】 Yang Junye;Xu Jiangou;Yang Shirou Research Unit of Neurophysiology

【机构】 华西医大神经生理研究室; 华西医大神经生理研究室;

【摘要】 作者取健康、活泼的Wistar大鼠10只,分为两组:一组5只,用生理多导记录仪记录皮层电图、运动电图、眼动图和肌电图,作为划分睡眠时相的客观指标;另一组5只,只记录皮层电图和运动电图,作为划分指标。结果表明:额-顶近中线皮层区的皮层电图具有一定的特征;两组睡眠多导图划分题眠时相的特点相同。作者认为,同时记录皮层电图和运动电图,即可划分睡眠各时相,简化了大鼠睡眠多导图的描记方法。

【Abstract】 Experiments were performed on 10healthy Wistar rats with chronically im-planted electrodes for the recording ofelectrocoticogram (ECoG), electro--ocu-logram (EOG ), and electro-myogram(EWG). All the rats were prepared for theimplantatiou of recording electroded under5% Ketamine (100mg/kg i.p. )anesthesia.Four to six days after operation, the ratwas put in an apparatus which was de-signed specifically sensitive to detect themovement of the animal without electrodeon the body so that electroactogram (EAG)could be recorded readily. One group ofexperiment animal (n = 5), recorded ECoG,EAG, EOG and EMG simultaneously, wascompared to another group(n = 5), recordedECoG and EAG simultaneously. The resultsof experiments indicate that. the ECoG.recorded from the the mesial frontal andoccipital areas, have certain characteris-tics during different sleep phases, and thecharacteristics for classifying sleep phasesby the two kinds of somnopolygram aresimilar. Therefore, simultaneous record-ing of the ECoG and EAG can be used todiscriminate the sleep phases in the rat.In this way,the sonmopolygraphic record-ing method for studying sleep in the ratwould be simplified.

【关键词】 睡眠; 慢波睡眠; 异相睡眠; 睡眠多导图;
【Key words】 Sleep; Slow wave sleep; Paradoxical sleep; Somnopolygram;
  • 【被引频次】1
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