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气相色谱法快速测定分支杆菌耐药性研究

Studies of Rapid Detection of Drug-Resistance of Mycobacterium by Gas Chromatography

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【作者】 何宗宁; 李景吾; 张素芳; 丁世宁; 吴良行; 赵庆昌;

【Author】 He Zhongning;Li Jingwu;Zhao Sufang;Ding Sining;Wu Liangxing;Zhao Qingcang Department of Internal Medicine school of Stomatology Sichuan University

【机构】 华西医大内科学教研室; 华西医大口腔医学院; 四川大学;

【摘要】 作者根据分支杆菌在生长代谢过程中产生CO2的特点,采用气相色谱法测定了肺结核及可疑肺结核患者78例的分支杆菌临床分离株在改良罗氏培养基中的CO2含量,借以判断分支杆菌的耐药情况,并用常规方法作为对照。结果表明:两法符合率为83.3%,气相色谱法能比常规方法提前20天出结果。

【Abstract】 This test ls based on the phyiologiccharaeter of mycobacterium,which produ-ces carbon dioxide(CO2)during its growth.Lowenstein-Jensen media CO2 contents-were measured by gas chromatographyfor datermining drug-resistance of myco-bacteria.This was compared with the rou-tine method. Seventy-eight mycobacteriawere isolated from suspected tuberculosispatients for the experiment.Our data sug-gested that the accordance rates of bothmethods were 83.3%. Gas chromatographyis a simple,rapid and accurate method fordetecting drug-resistance. The resultscan be obtained twenty days earlier thanby the routine method.

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